Non

Scientific Reports volume 13, Article number: 2370 (2023) Cite this article 1815 Accesses 2 Altmetric Metrics details The study was aimed to evaluate the performance of a newly developed

Scientific Reports volume 13, Article number: 2370 (2023) Cite this article

1815 Accesses

2 Altmetric

Metrics details

The study was aimed to evaluate the performance of a newly developed spectroscopy-based non-invasive and noncontact device (SAMIRA) for the simultaneous measurement of hemoglobin, bilirubin and oxygen saturation as an alternative to the invasive biochemical method of blood sampling. The accuracy of the device was assessed in 4318 neonates having incidences of either anemia, jaundice, or hypoxia. Transcutaneous bilirubin, hemoglobin and blood saturation values were obtained by the newly developed instrument which was corroborated with the biochemical blood tests by expert clinicians. The instrument is trained using Artificial Neural Network Analysis to increase the acceptability of the data. The artificial intelligence incorporated within the instrument determines the disease condition of the neonate. The Pearson’s correlation coefficient, r was found to be 0.987 for hemoglobin estimation and 0.988 for bilirubin and blood gas saturation respectively. The bias and the limits of agreement for the measurement of all the three parameters were within the clinically acceptance limit.

Neonatal jaundice, anemia and hypoxia are the most common health issues encountered by newborns globally and constitute a major percentage of infant mortality. The prevalence of neonatal jaundice, anemia and hypoxia is quoted to be between 50 and 60% among healthy term neonates1,2,3. According to the recent reports of World Health Organization, neonatal jaundice affects one in every two infants globally. One of the major reasons for pathologic hyperbilirubinemia is the excessive production of bilirubin, a byproduct of hemoglobin breakdown, and the impaired ability of the newborn to excrete it4. Among the reported cases of neonatal hyperbilirubinemia, about 15% of the neonates suffer from persistent jaundice that lasts for about 14 to 21 days5. All these infants suffering from persistent jaundice have significantly decreased hemoglobin levels6 and elevated bilirubin concentration in blood due to the increased bilirubin production from hemolysis resulting in a simultaneous pathologic condition of jaundice and anemia among neonates4,6. According to the American Academy of Pediatrics (AAP), the incidence of neonatal hyperbilirubinemia is increased among infants having risk factors like, ABO incompatibility, Rh incompatibility, birth asphyxia, etc.7. It has been reported that the occurrence of neonatal jaundice is more likely among neonates suffering from birth asphyxia, than neonates without birth asphyxia8,9,10,11 due to the lack of oxygen supply to the liver which results in hypoxic damage followed by the bilirubin conjugation ability of the liver, which ultimately results into jaundice8. Additionally, perinatal asphyxia and hypoxic-ischemic encephalopathy can lead to the disruption of the blood–brain barrier, allowing the free entry of unconjugated bilirubin to the neurons resulting in acute bilirubin encephalopathy8. In addition to this, dysregulation of blood flow to the lungs due to hemolysis can also cause an imbalance in the ventilation and perfusion ratio thereby, resulting in a hypoxic condition12 in the neonates13. Thus, simultaneous monitoring of bilirubin, hemoglobin and oxygen saturation levels in newborns is essential to ensure appropriate management. The contemporary method of measurement of serum bilirubin concentrations (TSB) hemoglobin levels (Hb) and arterial blood gas (for measurement of blood oxygen saturation) involves painful blood sampling14,15,16,17 which suffers from multiple long term consequences like infection at the sampling site, osteomyelitis (though in rare cases), blood loss, etc.15,18,19. Although, non-invasive methods (BiliCheck™20, JM-105™21, Rad 57™22 NBM-200, etc.)23 have been established as alternatives to repeated blood samplings for TSB and Hb measurement24,25 however, they suffer from certain inherent limitations that restrict their usage in widespread hospital settings24,26,27. Particularly, the accuracy of these non-invasive devices vary across races and have been found to be less accurate in Asian, Hispanic and African populations (having dark skin tone)28. On the other hand, the available transcutaneous pulse oximeters are the state-of-the art technology for the continuous estimation of oxygen saturation among neonates. Although, the pulse oximeters are correlated with the blood oxygen saturation and has been used in the hospital setting for more than a decade now, they suffer from certain limitations which may be improved to increase their acceptability. The pulse oximeters are vulnerable to the motion of the subject and, gives erroneous results by interpreting the motion of the infant as a pulse signal29. Moreover, these pulse oximeters overestimate the arterial oxygen saturation (SpO2) at less than 90% saturation thereby, limiting their usage in infants suffering from heart disease30,31. Therefore, monitoring of bilirubin, hemoglobin and SpO2 values is needed at the bedside of the neonate suffering from either jaundice, anemia or hypoxia for the proper management.

In this study, we aim to develop a non-invasive point of care device (i.e. SAMIRA, Spectrum Assisted Medical Inoffensive Radiation Application) for the simultaneous determination of hemoglobin, bilirubin and oxygen saturation in neonates. The newly developed instrument utilizes an algorithm to quantify three blood parameters from a single discrete measurement. The simultaneous measurement of bilirubin, hemoglobin and oxygen saturation from neonates in a non-invasive manner from a single measurement for their proper management is the motivation of the current work. As the device collects data from the blood vessels of the distal subungual arcade and the superficial arcade area discarding the influence of melanin or skin colour, it is hypothesized to give accurate transcutaneous measurement values of TSB, Hb and SpO2. Additional incorporation of machine learning and artificial intelligence has improved the data accuracy of the proposed device. By virtue of the machine learning algorithm the developed device is capable of locking a data which is 98% accurate. Studies on a huge number of neonatal populations helped in exact incident light dosage determination, data acquisition time optimization etc., making the device highly precise and accurate for point of care settings. Till date, to the best of our knowledge no device has been developed that can monitor these three blood parameters at the same time in a non-invasive way.

The instrumental set up as shown in Fig. 1a has been designed based on the principle of diffused reflectance spectroscopy. The diffused reflectance spectroscopy (DRS) technique is based on the amount of light collected from the tissue layers after penetration of the incident light32,33,34. The retro-reflected light thus contains information about the tissue microstructure and the biomolecular content. The thumb nail plate of neonatal subjects is illuminated with light from a white Light Emitting Diode (LED) source (3 W, 400–700 nm, 700 LUX, 4.78 mW optical power) and the diffused optical signal in the visible range was collected in a CCD based spectrograph (STS-VIS, manufactured by Ocean Optics, Florida) with wavelength resolution of 0.47 nm. A lab grade 6:1 diffuse reflectance fiber optic probe manufactured by Ocean Optics, Florida was used to transmit the light from source to subject nail by the 6 peripheral excitation fibers of the probe and receive the response signal by the central collection fiber by holding the probe tip at perpendicular position with the nail. The obtained spectral response in the spectrometer is then transferred to a computer through USB connection for processing in our developed graphical user interface in LabVIEW (National Instruments) platform. Subsequent data acquisition, analysis and result generation are taken care of by the custom-made software. Proper cooling arrangements (5 V DC fans, 0.2 Amp) for dissipation of excess heat generated by components have also been incorporated in the instrument by virtue of which the device performance remain unaltered under varying temperature conditions. In the customized power supply module driven device, artificial intelligence is also implemented by the software automatically to filter out reliable spectrum after data acquisition by guiding the user to take more data unless appropriate accuracy level is achieved. Figure 1b reveals the distinct difference in the spectral signature of blood for a control neonate (TSB of 2.4 mg/dL, Hb of 19.1 gm/dL and arterial blood gas of 95%) in comparison to a sick neonate (TSB of 29.8 mg/dL, Hb of 10.2 gm/dL and arterial blood gas of 92%).

Design of the developed prototype with the acquired data and the de-convoluted spectrum. (a) The instrument consists of a LED source, a 6:1 optical fibre, a CCD based spectrophotometer and an integrated electronic module (See text for details). (b) The acquired spectra from two subjects. Subject 1 is diseased with TSB value 29.8 mg/dL; Hb value 10.2 gm/dL; and SpO2 value of 92%. Subject 2 is from a normal infant with TSB value 2.4 mg/dL; Hb value 19.1 gm/dL and SpO2 value of 95%. (c) Work flow of the instrument (d) De-convoluted peaks from subject 1 along with the cumulative fit (e) De-convoluted peaks from subject 2 along with the cumulative fit (see text).

A graphic user interface was developed using LABVIEW software (National Instruments) for data acquisition, data analysis and generation of subsequent results35,36. The algorithm for the work flow of the device is shown in Fig. 1c. The whole blood spectrum obtained from the neonate’s nail bed was de-convoluted to obtain five independent signals at five wavelengths (462.92 nm, 539.34 nm, 568.09 nm, 577.2 nm and 620 nm). A comparative spectral response between a jaundiced and anemic infant (Subject 1) and a normal (Subject 2) and their de-convoluted spectra is shown in Fig. 1d,e.

The present study uses a sophisticated machine learning (ML) algorithm known as Artificial Neural Networks (ANN) to analyze the vast amount of data-set collected during the study. The main objective is to introduce artificial intelligence (AI) framework through machine learning (ML) techniques, which are dedicated algorithms to train the software to learn from data37.

The ANN algorithm tries to imitate the network of a human brain by learning tasks and solving problems38. The input layers and output layers of the network are connected by single or multiple hidden layers and interconnecting nodes with variable ‘weight factor’39.

Utmost care was taken for spectrum data acquisition through the developed prototype. Figure 1c illustrates the simple sequential program flow diagram of the software for accurate assessment of blood hemoglobin, bilirubin and oxygen saturation levels in neonates. After getting powered up, health check-up and initialization of the instrument takes place. If there is any discrepancy, the device auto-corrects different conditions and restarts automatically, followed by a pop-up window asking for patient details including name, age, sex, medical conditions etc. to be saved along with data in individual folders. The software next guides to store ‘reference’ and ‘dark’ spectra one time for a particular ambient condition. The dark spectrum was acquired in the presence of ambient light by turning off the source LED. The effect of light scattering from the nail bed was taken into account by recording the reference spectrum. The reference spectrum was collected using a standard scatterer (WS-1 Reflectance Standards, Ocean Optics) with a spot size of 0.7 cm in diameter. The distance between the probe tip and the scatterer was maintained perpendicularly at a distance of ~ 1.5 cm, such that the maximum light was scattered. The pre-acquired dark spectrum and reference spectrum, (which were acquired each day before starting the data collection) were read from the preloaded file location for spectrum processing. It is to be noted that the dark and reference spectra were acquired each day before starting the data collection to avoid the potential effects of variation in ambient light. In case of a change in the measurement location both spectra were re-acquired. The integration time of the spectrometer was kept fixed at 3000 ms and the boxcar width (Smoothening factor/running average) at 2 in this entire study for maintaining a proper signal to noise ratio (S/N) of the spectra. The average time to acquire reliable data using the device is approximately 30 s, whereas the time required to communicate the acquired data to the cloud is nearly 1 min.

For data processing mechanism, the following structure describes the various layers of ANN used for the present study (Fig. 2).

Machine Learning Algorithm (a) Schematic representation of ANN used in the proposed device for the detection of Hemoglobin, Bilirubin and Oxygen Saturation (b) Time dependent continuous series flow diagram to estimate the blood parameters in regular time intervals (see text).

The dark, reference, and sample spectra containing the absorbance values from 450 to 650 nm are the primary elements used by the input layer.

This layer generates the processed spectrum data using the following equation

The recording of the dark and reference spectra and the processing of the acquired signal in accordance to Eq. (1), compensates the impact of light scattering from the neonate’s nail bed. A training statement was also introduced in this layer which limits the amplitude of the absorbance range within 0.5 and 0.6 at 620 nm. This was maintained by the following instruction: 0.5 ≤ Abs 620 nm ≥ 0.6. This condition was given in order to maintain the spot size to be 0.7 cm and the distance between the probe tip and nail bed to be 0.6 cm.

Layer for de-convolution of the processed spectrum (raw signals) and assessing the residuals (amount of scattered data from the fitted line) of the fit parameters to be acceptable by the system. The whole blood spectrum collected from the neonates’ nail bed was de-convoluted into five Gaussian functions (Fig. 1d,e). The equation for each Gaussian function (y) is as follows:

where y0 is the offset, A is the amplitude of the Gaussian curve, w is the full width by half maxima (FWHM) and xc is the peak wavelength of the Gaussian curve. Each of the five Gaussian curves has a fixed peak wavelengths at 462.92 nm, 539.34 nm, 568.09 nm, 577.2 nm and 620 nm. The peak wavelengths were chosen on the basis of the pattern of absorption of oxygenated hemoglobin, de-oxygenated hemoglobin and of bilirubin.

This layer is also responsible for generation of membership functions, where the peak wavelength of 462.92 nm corresponds to the absorption of bilirubin, 539.34 nm and 577.2 nm corresponds to the Q bands of oxy-hemoglobin and that of 568.09 nm corresponds to the de-oxygenated peak of hemoglobin. The additional peak at 620 nm has been considered to correct the baseline and scattering contributions from the acquired spectrum.

In this layer a cumulative fit of the data was procured by adding each of the independent five Gaussian curves as follows.

where A1 A2, A3, A4 and A5 is the amplitude of the Gaussian curves with peak wavelengths at 462.92 nm, 539.34 nm, 577.2 nm, 568.09 nm, and 620 nm respectively, as mentioned earlier. In this layer, the area under each of the Gaussian curves is calculated using the Trapezoidal Rule, using the cumulative fitted equation. Assuming f(x) to be continuous over [a,b], the area under each of the Gaussian curve has been calculated using,

The instrumentation indices for the parameters were identified in this layer using the area under the five Gaussian curves and appropriate weightage was given to each one of them. It was noted that, summation of the area under the curves (AUC) corresponding to the wavelength of 539.34 nm and 577.2 nm (AUC539.34 + AUC577.2) provide an estimation of haemoglobin as the absorption band at 539 nm and 577 nm of the blood spectrum corresponds to the absorption of oxygenated hemoglobin and the trough at 568 nm corresponds to the de-oxygenated hemoglobin40,41. Similarly, bilirubin and oxygen saturation were calculated by measuring the AUC462.92 (as 462 is the characteristic peak of bilirubin42) and AUC577.2/AUC568.09 respectively. The amplitude of the Gaussians changed in relation to the amount of the biomolecule (bilirubin and hemoglobin) present. The change in the AUCs was mapped with the gold standard to obtain the calibration curve.

In this layer the residual number of points of the fitted data from the acquired spectrum was analysed. The amount of the scattered data from the acquired signal with respect to the pre specified parameters given in hidden layer L3 is analysed in this layer. For the acceptability of the data, 98% of the residual data points necessarily must be within the range of ± 0.02% from the cumulative fitted curve. On the contrary, the scattered data points beyond ± 0.02% will be rejected. A comparison between the accepted data and the rejected data is shown in Fig. 3a–f.

Training sets of the device using the self-devised algorithm (a,c,e) Raw and fitted data with the scattered residual points between ± 0.02% from the cumulative fitted data, used for the estimation of TSB (19.8 mg/dL), Hb (11.2 gm/dL) and SpO2 (99%). (b,d,f) Raw and fitted data with the scattered residual points beyond ± 0.02% from the cumulative fitted data, discarded by the algorithm for the estimation of TSB (19.8 mg/dL), Hb (11.2 gm/dL )and SpO2 (99%). (g–i) The calibration curve between the instrument index values acquired from the device with the obtained TSB values from blood test at the three partitions (see text) in 229 neonates respectively. The calibration curve shows a polynomial nature with the TSB values and the instrument index function. (j–l) The calibration curve between the instrument index values acquired from the device with the obtained Hb values from blood test at the three partitions (see text) in 1072 neonates respectively. The calibration curve shows a linear dependency with the Hb values and the instrument index function. (m–o) The calibration curve between the instrument index values acquired from the device with the obtained ABG values from biochemical test at the three partitions (see text) in 483 neonates respectively. The calibration curve shows a polynomial nature with the ABG values and the instrument index function.

Initially, the machine was trained to accept the spectra, when 90% of the residual data points of the fitted data were within the range of ± 0.02%. The Adjusted r2 for quantification of hemoglobin, bilirubin and oxygen saturation (Table 1) was found to be 0.77, 0.96 and 0.95 respectively for this training data set (Fig. 3g). Thus, a library containing the hemoglobin, bilirubin and oxygen saturation values of 483 neonates was prepared. The prototype-specific instrument indices were clinically validated using a regression analysis (Fig. 3)43,44. The minimization problem was utilized to minimize the error between the predicted value and the actual value using the following equations

where, J is the minimization function. The difference between the predicted values and the acquired values measures the error difference.

The Mean Squared Error (MSE) function over all the data points, has been computed by squaring the error difference and summed over all data points and divided that value by the total number of data points.

To reduce the error the machine was trained to accept the spectra, when 95% of the residual data points of the fitted data were within the range of ± 0.02%. The Adjusted r2 for quantification of hemoglobin, bilirubin and oxygen saturation was found to be 0.96, 0.97 and 0.95 respectively for this training data set (Fig. 3). However, the standard deviation was ± 5.4 for the estimation of the three blood parameters. Finally, the machine was trained to accept the spectra, when 98% of the residual data points of the fitted data were within the range of ± 0.02%. The Adjusted r2 for quantification of hemoglobin, bilirubin and oxygen saturation was found to be 0.96, 0.97 and 0.95 respectively for this training data set (Fig. 3). The hidden layer L5 basically identifies good data and bad data, through the error calculation. Figure 3a–f shows clearly, how the good data is remarkably different from bad data in terms of the amount of scattered data from the cumulative fitted line.

The processed data in a data library are deposited locally as well as in the cloud-storage. Provision of a dynamic calibration library strengthens the overall algorithm of the device by iterative method.

This layer is responsible for decision making protocol as well. Identification of a good data leads to output layer, where blood report would be generated, whereas, for bad data, back-propagation technique would be adopted.

Output layer estimated the hemoglobin, bilirubin and oxygen saturation of the neonates and displayed them. Consequently, it also displayed whether the neonate is suffering from, either anemia, jaundice and hypoxia. The IOT enabled device enables its user to send blood reports via email and SMS. If anemia is detected, the online report may be dispatched to the doctor or patient party to expedite the treatment procedure. Finally, a dialog box appears to ensure that whether the job is to be terminated or repeated. The easy and user- friendly operating software interface makes the device to be handled by any layman without any prior medical or instrumentation knowledge.

The developed prototype can detect the blood parameters in equal time intervals and is suitable for time series monitoring governed by the following equation:

For the neonates undergoing phototherapy, the equal interval time series analysis would be compared with the Bhutani nomogram7, to detect the risk level associated with infant's hours of age and serum bilirubin concentration. For haemolytic anaemic patients and neonates suffering from heart diseases or hypoxia, time dependent data monitoring of haemoglobin and oxygen saturation will be crucial for treatment management and to determine the future courses of therapeutic action.

The experimental protocol consisted of the data collection using the in-house device (SAMIRA), parameters were calculated from the data. The measurements using the device was recorded at the same time blood was collected from neonate. The blood was sent for analysis to estimate the TSB, Hb and SpO2, which was corroborated by a statistician blinded to the entire study. A single measurement was taken from the neonate’s thumb nail bed to estimate three blood parameters simultaneously.

This was a prospective observational study conducted over 25 months starting from January 2017 at the Department of Pediatric Medicine, Nil Ratan Sircar Medical College and Hospitals (NRSMH, a Govt. aided tertiary hospital), Kolkata, India.

The sample size was estimated using the Everald’s equation for power calculation in diagnostics tests45. Assuming the expected lowest sensitivity (SN) to be 95%, lowest expected specificity (SP) to be 80%, confidence interval (W) for both sensitivity and specificity to be 5% and prevalence of neonatal jaundice, anemia and hypoxia to be 15%2,3,46 the minimal sample size required to achieve the targeted sensitivity and specificity were found to be 487 and 290 each respectively. Hence, the effective population size is 2331. However, we decided to include a much higher number (N = 4318) of subjects in our study to reach a more robust statistical outcome. Out of this, 3427 subjects were analyzed using our developed algorithm. The remaining 891 subjects were excluded on the basis of our proposed algorithm.

The study included 4668 neonates with gestational age from 28 to 40 weeks. Among them, 70 subjects failed the recruitment criteria, of which 47 subjects had cannula on either of the hands and 9 patients had other complications (e.g., inaccessible thumbnail, uneven nail bed, or other physical problems with the thumb), and were thus excluded. Based on deteriorated blood samples (hemolysed blood samples, delayed blood processing, inadequate blood volume, and ambiguous blood information), 223 patients were further ruled out from the analysis. Hence, the effective population size of 4318 neonates was considered for the study. Comprehensive details of the subjects are provided in Table 2. Out of this, 3689 subjects were analyzed using our developed algorithm. The remaining 630 subjects were excluded on the basis of our proposed algorithm. Particulars about inclusion and exclusion criteria are described in Table 3. Out of the 3689 neonates, measurements from 1784 subjects were used for the training or calibration of the device. The rest of the 1935 neonatal subjects were selected for the validation of the instrument.

It is worth mentioning that the recruitment of neonates was not consecutive as not all physicians practicing in the department were involved in the study. The neonates getting treatment under the physicians associated with the study were inducted. The appearance of possible selection bias was avoided following the approach described by Hammer et al.47. Random assignment of doctors (a general policy for the public hospitals in India), large time frame of the study (15 months), sufficiently large sample size, collection of data throughout 24 h window, and enough number of subjects in each subcategory (i.e., stratification of samples) helped in avoidance of the sampling bias.

Care was taken that a similar clinical protocol i.e., study, reference, and sample collection methods, and patient enrolment strategies were prospectively maintained throughout the experimental period. To avoid bias in measurements, particular care was taken to keep the technicians, clinicians, investigators, and data analysts at data collection sites blinded to the SAMIRA and the hematological data. Data of each neonate on pre-defined variables like the date, identification number, sex, gestational age, maternal history, whether having any risk factors, treatment details, etc. was collected from clinical charts on a tablet having required database with the in-built proforma by one laboratory technician hired for the study purpose. Blood collection, serum isolation, and measurements by SAMIRA were performed by trained nurses of the Department of Pediatric Medicine, NRSMH. They were responsible for uploading the SAMIRA readings to the database. The hematological parameters were measured by expert clinical biochemists at Central Laboratory, NRSMH who were completely unaware of the study. The TSB, Hb and arterial blood gas readings with proper identification numbers of the selected subjects were uploaded by another laboratory technician hired for the study purpose. The readings of both the methods (SAMIRA and the conventional) were matched based on the identification number by one research staff, to ensure complete blindness of the study. Complete blinding was maintained to keep the two sets of readings separate.

For simultaneous measurement, about 2 mL of blood was collected for the conventional TSB, Hb and arterial blood gas measurement test within 30 min of the data collected from SAMIRA.

The TSB of the subjects was quantitatively determined by the 2,5-dichlorophenyldiazonium tetrafuoroborate (DPD) diazo method described by Jendrassik and Groff48, using the commercially available test kit (Autospan Liquid Gold, Span Diagnostics, India) within 1 h of blood collection in the Central Laboratory, NRSMH. For the test, serum was first isolated from the collected blood and then examined with the test kit. To prevent the photoreduction of bilirubin the serum samples were carefully kept in the dark at 4 °C before analysis.

For hemoglobin and arterial blood gas measurement the collected blood samples were subjected to an automated hematology analyzer (Sysmex KX-21)49 for complete blood count (CBC) analysis and GEM premier 3000 system50 (Instrumentation Laboratory. Bedford, MA), respectively.

All the guidelines provided by the National Accreditation Board for Testing and Calibration Laboratories (NABL)51 were followed to maintain the accuracy and precision of the techniques. The coefficient of variation for the hospital laboratory was targeted for < 6%. During the study period, each of the actual variance values, assessed every 3 months, ranged from 3 to 5%.

Analysis of the data was done using descriptive statistical analysis, simple linear regression analysis, and the Bland & Altman method52,53,54,55. For the correlation between the values obtained from the device and the gold standard, linear regression and Bland Altman was used. The mean (n) of the measurements was calculate according to the formula:

The Standard deviation between the successive measurements was measured using the formula

where, N refers to the number of experimental outcomes, µ is the mean of the individual outcomes and xi is each outcome of the experiment. GraphPad Prism 5.0 (GraphPad Sofware, USA) and SigmaPlot 12.5 (Systat Sofware, USA) were utilized for the analysis of the data.

For the present work, all necessary ethical permissions were taken from the Institutional Medical Ethics Committee, NRSMH, Kolkata (Ref. No.—No/NMC/439, dated January 27, 2020). All studies involving human subjects were performed following the Declaration of Helsinki56 and guidelines provided by the Indian Council for Medical Research (ICMR), Govt. of India. Written informed consent was obtained from parents or legal guardians who agreed to participate in the study after understanding the details of the study and its consequences. All data and information about the subjects were anonymized, kept confidential and used only for this study.

In order to acquire the optimum condition for data acquisition, we performed data analysis using the self-devised algorithm in 3 different partitions on the calibration dataset. The dataset was divided into 3 partitions depending on the amount of scattered data points from the cumulative fitted curve. The three partitions are 90% (when 90% of the data points are within the range of ± 0.02); 95% (when 95% of the data points are within the range of ± 0.02) and 98% (when 98% of the data points are within the range of ± 0.02).

The correlation plot of the instrument at the three partitions with the three blood parameters calculated from the standard biochemical test on 1784 neonatal subjects shows three patterns of dependency on the blood parameters (Fig. 3g–o). Figure 3g–i shows the second order polynomial dependency of the instrument index values with TSB levels for all the three partitions. The Adjusted r2 was found to be as follows: when 90% of the data points are within the range of ± 0.02, 0.77. When 95% of the data points are within the range of ± 0.02, the adjusted r2 is 0.96 and when 98% of the data points are within the range of ± 0.02, the adjusted r2 is 0.97. The instrument index values maintain a linear relationship with the Hb values obtained from blood tests for all the partitions (Fig. 3j–l). The Pearson’s correlation coefficient, r was found to be 0.969 (slope = 1.7622; intercept = 2.862) when 90% of the data points are within the range of ± 0.02. When 95% of the data points are within the range of ± 0.02, correlation coefficient, r was found to be 0.972 (slope = 1.911; intercept = 1.75) and when 98% of the data points are within the range of ± 0.02, the correlation coefficient, r was 0.9745 (slope = 1.8; intercept = 2.91). Moreover, a fourth order polynomial dependency of the instrument index values was obtained with the arterial blood gas for all the three partitions of the datasets (Fig. 3m–o). The Adjusted r2 was found to be as follows: when 90% of the data points are within the range of ± 0.1. is 0.953; When 95% of the data points are within the range of ± 0.05, the adjusted r2 is 0.974 and when 98% of the data points are within the range of ± 0.02, the adjusted r2 is 0.989.

A total number of 1935 neonates were selected for validation of the instrument (Fig. 4). The instrument was validated in 409 subjects suffering from neonatal jaundice. 65 anemic subjects, and 223 subjects suffering from hypoxia. The remaining 1238 subjects were taken as control population for the purpose of validation of the device. All the datasets were divided into three partitions and the analyzed using the developed algorithm. Linear regression and Bland–Altman analysis was performed to evaluate the correlation between the obtained data produced by the instrument with the gold standard clinical laboratory tests.

Validation of the instrument in different partitions of the dataset. Linear regression plot of the developed device verses TSB on 230 neonates, when (a) 90% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (d) 95% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (g) 98% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. Linear regression plot of the developed device verses Hb on 1073 neonates, when (b) 90% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (e) 95% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (h) 98% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. Linear regression plot of the developed device verses SpO2 on 340 neonates, when (c) 90% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (f) 95% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (i) 98% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot.

Linear regression analysis between the values obtained from the instrument at the three partitions with the blood tests show a more correlation when 98% of the data points are within the range of ± 0.02 (for TSB estimation, r = 0.988; slope = 1.006; intercept = 0.372; for Hb estimation, r = 0.987; slope = 0.954; intercept = 0.668; for estimation of oxygen saturation, r = 0.988slope = 0.977 ; intercept = 1.775 , Fig. 4g–i) in comparison when 95% of the data points are within the range of ± 0.02 (for TSB estimation, r = 0.975; slope = 0.94; intercept = 0.844; for Hb estimation, r = 0.969 slope = 0.917; intercept = 1.211; for estimation of oxygen saturation, r = 0.985; slope = 1.029; intercept = 2.098, Fig. 4d–f). On the contrary, when 90% of the data points are within the range of ± 0.02 the correlation was reduced (for TSB estimation, r = 0.894; slope = 0.852; intercept = 2.564; for Hb estimation, r = 0.9783; slope = 0.911; intercept = 1.1749; for estimation of oxygen saturation, r = 0.975 slope = 0.961; intercept = 2.375, Fig. 4a–c). Bland–Altman analysis (Fig. 5) also corroborated the highest correlation between the when 98% of the data points are within the range of ± 0.02 and the obtained blood parameter values of TSB, Hb, and arterial blood gas for both normal and diseased neonatal subjects in comparison to the other two partitions. The statistical parameters when 98% of the data points are scattered within a range of ± 0.02 are as follows: with the TSB values obtained from the biochemical test, (bias for normal = − 0.491 mg/dL, jaundiced subjects = 0.099 mg/dL; 95% limits of agreement for normal = − 2.37 mg/dL to 1.3 mg/dL and jaundiced subjects = − 1.98 mg/dL to 1.71 mg/dL, Fig. 5c,l); with Hb values obtained from the blood tests (bias for normal; − 0.08 gm/dL, anemic; 0.19 gm/dL; 95% limits of agreement for normal; − 3.43 gm/dL to 3.10 gm/dL, anemic; − 2.04 gm/dL to 2.07 gm/dL and − 0.93 gm/dL to 1.23 gm/dL, Fig. 5f,o) and with the arterial gas values (bias for normal = − 0.56% and hypoxic = − 1.05%; 95% limits of agreement for normal = − 2.38% to 1.38% and hypoxic = − 3.45% to 1.6%, Fig. 5i,r). The statistical parameters when 95% of the data points are within the range of ± 0.02 are as follows: with the TSB values obtained from the biochemical test, (bias for normal = 0.25 mg/dL, jaundiced subjects = − 0.11 mg/dL; 95% limits of agreement for Normal = − 2.56 mg/d to 3.13 mg/dL, Jaundice subjects = − 2.7 mg/dL to 2.62 mg/dL Fig. 5b,k); with Hb values obtained from the blood tests (bias for normal = − 0.3104 gm/dL anemic = 0.4952 gm/dL; 95% limits of agreement for Normal = − 5.17 gm/dL to 4.55 gm/dL, Anemic = − 2.36 gm/dL to 3.35 gm/dL Fig. 5e,n) and with the arterial gas values (bias for Normal = 1.13%, Hypoxic = − 0.23%; 95% limits of agreement for Normal = − 4.49% to 2.64%, Hypoxic = − 4.16% to 4.32% Fig. 5h,q). However, when the 90% of the data points are within the range of ± 0.02, the statistical parameters are as follows: with the TSB values obtained from the biochemical test, (bias for normal = 0.37 mg/dL, jaundiced subjects = 0.153 mg/dL; 95% limits of agreement for normal = − 2.85 mg/dL to 4.2 mg/dL and jaundiced subjects = − 6.39 mg/dL to 6.56 mg/dL, Fig. 5a,j); with Hb values obtained from the blood tests (bias for Normal = − 0.43 gm/dL, Anemic = 0.91 gm/dL; 95% limits of agreement for Normal = − 8.14 gm/dL to 7.26 gm/dL, Anemic = − 3.71 gm/dL to 5.54 gm/dL, Fig. 5d,m) and with the arterial gas values (bias for Normal = − 1.05%, Hypoxic = − 1.4%; 95% limits of agreement for Normal = − 6.56% to 4.17%, Hypoxic = − 4.78% to 3.83% Fig. 5g,p). 95% limits of agreement means that 95% of the differences are assumed to lie within these limits and how far apart the measurements obtained using the two methods are likely to be for most individuals. Bias refers to the difference between the expected value obtained from the device and the true value of the parameter being obtained from the haematological tests. Detailed results of comprehensive statistical analysis are presented in Table 4.

Relationship between the developed device and the blood parameters obtained from blood sampling in normal neonates (Control population) and diseased neonates. Bland–Altman plots (mean and 95% limits of agreement) between the developed device and TSB values in control and jaundiced subjects respectively when, (a,j) 90% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (b,k) 95% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (c,l) 98% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. Bland–Altman plots (mean and 95% limits of agreement) between the developed device and Hb values in control and anemic subjects respectively when, (d,m) 90% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (e,n) 95% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (f), (o) 98% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. Bland–Altman plots (mean and 95% limits of agreement) between the developed device and ABG values in control and hypoxic subjects respectively when, (g,p) 90% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (h,q) 95% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot. (i,r) 98% of the acquired data points are scattered within ± 0.02% from the cumulative fitted curve in the residual plot.

In order to check the repeatability of the device, measurement was taken from the neonate’s nail bed 5 times by the same observer. The repeatability of the device to parameterize the hemoglobin, bilirubin and oxygen saturation values was performed in 135 neonates (Fig. 6).

Repeatability of the data acquired using the developed device. Linear Regression analysis for five successive values of (a) Bilirubin (b) Hemoglobin (c) SpO2 measurement on the same subject by the same observer. Bland–Altman analysis for five successive values of (d) Bilirubin (e) Hemoglobin (f) SpO2 measurement on the same subject by the same observer.

We found a standard deviation of 3.2 mg/dL between back-to-back measurements in the same subject by the same observer in the estimation of bilirubin. For the estimation of hemoglobin and oxygen saturation, the standard deviation values were found to be 4.6 gm/dL and 3.2% respectively. The calculated SD and mean were almost the same in both the measurements, for all the three blood parameters. The linear regression analysis between the two measurements further confirmed the accuracy of the two measurements. For the repeatability analysis, the correlation between the measurements are as follows: for TSB measurement, (r = 0.989; slope = 1.0124; intercept = 0.1815, P < 0.001), for the estimation of Hb (r = 0.96642; slope = 0.783; intercept = 2.95, P < 0.001), and for the measurement of SpO2 (r = 0.963; slope = 0.99, P < 0.001). Bland Altman analysis was performed to confirm the correlation between successive measurements; for TSB measurement (bias = 0.183 mg/dL, 95% limits of agreement = − 1.25 to 1.287 mg/dL) for Hb estimation (bias = − 0.85gm/dL 95% limits of agreement = − 9.2 gm/dL to 9.05 gm/dL) for estimation of oxygen saturation (bias = − 1.01% 95% limits of agreement = − 6.46% to 6.25%).

The conventional non-invasive trans cutaneous methods are yet to replace the invasive method of blood sampling due to certain shortcomings of the transcutaneous devices. The TcB (trans cutaneous bilirubin) measured by these non-invasive devices consists of a major contribution from the extravascular bilirubin, which is a completely a different physiological parameter in comparison to the TSB. The unpredictable process that regulated the dynamics of bilirubin in the extravascular space makes a one to one comparison of TSB and TcB impossible26,27. Confinement of the measurement volume only to the intravascular space could help in overcoming the problem27. Whereas, the Hb and the SpO2 values acquired by the available transcutaneous hemoglobin meters and pulse oximeters have a significant positive bias31,57. This in turn, causes improper management particularly to the diseased population that tend to get their Hb and blood saturation values over estimated. The SAMIRA device is based on such spectroscopy based approach where the information is collected from the vascular bed underneath the nail plate55. Thus, we hypothesize that SAMIRA will be able to overcome the limitations of the conventional trans cutaneous devices for the simultaneous measurement of three blood parameters from a single optical spectrum.

Our results suggests that the bilirubin, hemoglobin, and SpO2 values obtained from SAMIRA has a positive linear correlation with all the three blood parameters (for Hb measurement, r = 0.96; for bilirubin measurement, r = 0.98; for SpO2 measurement, r = 0.98). Using the self-devised algorithm and multivariate regression analysis, we observed that when 95% of the acquired data points are within the range of ± 0.02, the optimum condition for acquiring reliable data from the neonates was achieved. Furthermore, the demographic analysis (Table 2) showed that postnatal age, exclusive breastfeeding, gestational age or any other risk factor are not associated with any variation in the performance of the device. No bias of birth weight and/or gestational age was found to observe on the non-invasive blood parameter measurements in the intensive study on 4318 neonatal subjects. Moreover, as the study was conducted on a subgroup of Indian population, the interference of dark skin colour (or variation within the Indian subpopulation) can also be ruled out, as Indian population consists of mixed races with varied skin tones. Skin tone was not found to be a confounding factor for the estimation of Hb, TSB or SpO2. However, the device slightly overestimated the bilirubin values by 5 mg/dL when the TSB exceeded 15 mg/dL. Otherwise, it marginally overestimated the bilirubin values by 2 mg/dL. Although this may result in unnecessarily prolonged hospitalization, it eliminates the chances of serious clinical errors like mismanagement of a diseased infant and hence decreases infant morbidity and mortality.

The correlation coefficient between SAMIRA and the arterial blood gas measurements was found to be 0.98, which is more than the other pulse oximeter devices where the correlation coefficient is of the order of 0.8 to 0.8558. Furthermore, the correlation with SpO2 < 70%, was found to be 0.98, which was much higher than the other non-invasive pulse oximeters with a correlation coefficient of 0.8–0.8530, which tends to overestimate the SpO2values below 70%. The correlation coefficient between the developed device and the TSB values was found to be 0.88, which was better than the correlation shown other non-invasive devices which was of the order of 0.7–0.859,60. It has to be noted that these studies were conducted on white population in which the conventional TcB meters generally show good efficacy. Several studies have reported that TcB meters overestimate bilirubin values in dark skin populations like Hispanic, Asian, African, etc.24,28,61.In our study values of overestimation by ≥ 2 mg/dL, ≥ 3 mg/dL and ≥ 4 mg/dL were found to be 25%, 4.2% and 0.9% respectively. To the best of our knowledge, no TcB device has been developed to address bilirubin overestimation in the black population, and a low-cost, non-invasive, point of care device for these ethnic groups holds promise for low and middle income countries62. The correlation of the device in the anemic region (Hb < 11 gm/dl) was found to be 0.99, which was highly comparable with the commercially available devices with a correlation coefficient of 0.9522,23. It has to be noted that these available instruments estimated the hemoglobin values in adults. Due to the different architecture and thickness of the skin, adult hemoglobin meters are not a suitable comparison to the neonatal trans cutaneous hemoglobin meters. The Bland–Altman analysis confirmed that SAMIRA was highly sensitive in the anemic region, which suggests that even the mildest form of anemia can be detected by the device.

In some studies, it has been shown that pulse oximetry usually overestimates the SpO2 values less than 90% in cyanotic children31,63,64 However, the correlation between the developed device with the SpO2 values less than 90% is 0.99. The limits of agreement is between − 20.9% to 10.3%31 for the other pulse oximeters which shows, that each point suffers from huge standard deviation. Whereas, the limits of agreement are within − 4.3% to 5.3% of our developed device, which ensures lesser fluctuation of the data points.

This is one of the few studies that extensively assessed the performance of a non-invasive device in neonates suffering either from hyperbilirubinemia or anemia or hypoxia. We think that the strength of the study lies in the prospective design, a large number of sample in each category, the intra-racial variation in the dark skin color, the parallel measurement of three blood parameters (bilirubin, hemoglobin, arterial blood gas) by two methods (SAMIRA and the biochemical tests), and the collection of the blood for the routine biochemical tests by regular nurses for clinical use rather than specifically for the study purpose in which conditions might be optimized. The conventional measurements were performed by the experienced clinical biochemists and laboratory technicians of a tertiary care hospital, reducing the possibility of operator error. Overall, we believe that our results provide a robust estimation of the accuracy of non-invasive hemoglobin, bilirubin and SpO2 measurement by a new device and the sources of error are applicable to routine clinical settings.

Our study had few limitations over the other transcutaneous devices. The available transcutaneous instruments did not perform well on subjects having a bilirubin report of more than 15 mg/dL.65,66. In subjects where the bilirubin crossed 15 mg/dL, it was associated with severe complications like exchange transfusion and was avoided for complications. Another confounding factor of the study was that measurements on neonates with less than 500 g of body weight was not considered for the study due to their added complications. Studying the population having high TSB values and also associated with the risk factors for hyperbilirubinemia, anemia and hypoxia, and manipulation of the intensity of illumination can further increased the accuracy of the device. Lastly, the results obtained from the non-invasive measurements (SAMIRA) were used for clinical management. A real-time clinical management using these readings has helped to assess the actual accuracy of this device to reduce painful blood sampling in day-to day clinical practice.

The new non-invasive non-contact device (SAMIRA) can accurately measure hemoglobin, bilirubin and SpO2 levels simultaneously from a single optical spectrum. It could also measure TSB > 20 mg/dL, which was eliminated by the modification in the illumination intensity of the source. Interestingly, in this study we also found that the device gave accurate results to predict the onset of heart diseases in neonates by measuring their blood saturation levels. This will help clinicians to better monitor the neonates and reduce the frequency of blood sampling. Moreover, the device collects the data from the neonate’s nail bed which contains less melanin interference irrespective of the skin tone of the neonate. Overall, our results suggest that our device can be used in hospital settings for the accurate measurement of hemoglobin, bilirubin and SpO2 for the screening of hyperbilirubinemia, anemia and heart diseases in neonates.

Data that support the findings of this study are available with the corresponding author upon reasonable request. Figures 1, 2, 3, 4, 5 have associated raw data which is available to the corresponding author on request.

Code for the algorithm is available with the corresponding author and can be shared with the readers on request.

Brits, H. et al. The prevalence of neonatal jaundice and risk factors in healthy term neonates at National District Hospital in Bloemfontein. Afr. J. Primary Health Care Fam. Med. 10, 1–6 (2018).

Google Scholar

Onyeneho, N. G., Ozumba, B. C. & Subramanian, S. Determinants of childhood anemia in India. Sci. Rep. 9, 1–7 (2019).

Article CAS Google Scholar

Subhi, R. et al. The prevalence of hypoxaemia among ill children in developing countries: A systematic review. Lancet. Infect. Dis 9, 219–227 (2009).

Article PubMed Google Scholar

Dennery, P. A., Seidman, D. S. & Stevenson, D. K. Neonatal hyperbilirubinemia. N. Engl. J. Med. 344, 581–590 (2001).

Article CAS PubMed Google Scholar

Gilmour, S. M. Prolonged neonatal jaundice: When to worry and what to do. Paediatr. Child Health 9, 700–704 (2004).

Article PubMed PubMed Central Google Scholar

Alkhotani, A., Eldin, E. E. M. N., Zaghloul, A. & Mujahid, S. Evaluation of neonatal jaundice in the Makkah region. Sci. Rep. 4, 1–6 (2014).

Article Google Scholar

American Academy of Pediatrics. Management of hyperbilirubinemia in the newborn infant 35 or more weeks of gestation (Subcommittee on Hyper-bilirubinemia). Pediatrics 114, 297–316 (2004).

Article Google Scholar

Bizuneh, A. D., Alemnew, B., Getie, A., Wondmieneh, A. & Gedefaw, G. Determinants of neonatal jaundice among neonates admitted to five referral hospitals in Amhara region, Northern Ethiopia: An unmatched case-control study. BMJ Paediatr. Open 4, e000380 (2020).

Article Google Scholar

Devi, D. S. & Vijaykumar, B. Risk factors for neonatal hyperbilirubinemia: A case control study. Int. J. Reprod. Contracept. Obstet. Gynecol. 6, 198–203 (2017).

Article Google Scholar

Omekwe, D. E. et al. Survey and management outcome of neonatal jaundice from a developing tertiary health centre, Southern Nigeria. IOSR J. Dent. Med. Sci. 13, 35–39 (2014).

Article Google Scholar

Kolawole, S., Obueh, H. & Okandeji-Barry, O. Prevalence of neonatal jaundice in Eku Baptist community hospital in delta state Nigeria. J. Public Health Epidemiol. 8, 87–90 (2016).

Article Google Scholar

Nouraie, M. et al. The relationship between the severity of hemolysis, clinical manifestations and risk of death in 415 patients with sickle cell anemia in the US and Europe. Haematologica 98, 464 (2013).

Article CAS PubMed PubMed Central Google Scholar

Quraishi, H. & Chee, M. Pediatric Otolaryngology, An Issue of Pediatric Clinics of North America, E-Book Vol. 69 (Elsevier Health Sciences, 2022).

Google Scholar

Yap, S., Mohammad, I. & Ryan, C. Avoiding painful blood sampling in neonates by transcutaneous bilirubinometry. Ir. J. Med. Sci. 171, 188–190 (2002).

Article CAS PubMed Google Scholar

Meites, S. Skin-puncture and blood-collecting technique for infants: Update and problems. Clin. Chem. 34, 1890–1894 (1988).

Article CAS PubMed Google Scholar

Wang, L. et al. Non-invasive monitoring of blood oxygenation in human placentas via concurrent diffuse optical spectroscopy and ultrasound imaging. Nat. Biomed. Eng. 6, 1017–1030 (2022).

Article CAS PubMed Google Scholar

Yun, S. H. & Kwok, S. J. Light in diagnosis, therapy and surgery. Nat. Biomed. Eng. 1, 1–16 (2017).

Article Google Scholar

Lilien, L. D., Harris, V. J., Ramamurthy, R. S. & Pildes, R. S. Neonatal osteomyelitis of the calcaneus: Complication of heel puncture. J. Pediatr. 88, 478–480 (1976).

Article CAS PubMed Google Scholar

Lemont, H. & Brady, J. Infant heel nodules: Calcification of epidermal cysts. J. Am. Podiatr. Med. Assoc. 92, 112–113 (2002).

Article PubMed Google Scholar

Jangaard, K., Curtis, H. & Goldbloom, R. Estimation of bilirubin using biliChek™, a transcutaneous bilirubin measurement device: Effects of gestational age and use of phototherapy. Paediatr. Child Health 11, 79–83 (2006).

Article PubMed PubMed Central Google Scholar

Engle, W. D., Jackson, G. L. & Engle, N. G. Transcutaneous bilirubinometry. Semin. Perinatol. 38, 438–451 (2014).

Article PubMed Google Scholar

Hampson, N. B., Ecker, E. D. & Scott, K. L. Use of a noninvasive pulse CO-oximeter to measure blood carboxyhemoglobin levels in bingo players. Respir. Care 51, 758–760 (2006).

PubMed Google Scholar

Lamhaut, L. et al. Comparison of the accuracy of noninvasive hemoglobin monitoring by spectrophotometry (SpHb) and HemoCue® with automated laboratory hemoglobin measurement. J. Am. Soc. Anesthesiol. 115, 548–554 (2011).

Article CAS Google Scholar

Maisels, M. J. Transcutaneous bilirubin measurement: Does it work in the real world?. Pediatrics 135, 364–366 (2015).

Article PubMed Google Scholar

Maisels, M. J. & Kring, E. Transcutaneous bilirubinometry decreases the need for serum bilirubin measurements and saves money. Pediatrics 99, 599–600 (1997).

Article CAS PubMed Google Scholar

Maisels, M. J. Noninvasive measurements of bilirubin. Pediatrics 129, 779–781 (2012).

Article PubMed Google Scholar

Bosschaart, N. et al. Limitations and opportunities of transcutaneous bilirubin measurements. Pediatrics 129, 689–694 (2012).

Article PubMed Google Scholar

Wainer, S., Rabi, Y., Parmar, S. M., Allegro, D. & Lyon, M. Impact of skin tone on the performance of a transcutaneous jaundice meter. Acta Paediatr. 98, 1909–1915 (2009).

Article PubMed Google Scholar

Richards, N. M., Giuliano, K. K. & Jones, P. G. A prospective comparison of 3 new-generation pulse oximetry devices during ambulation after open heart surgery. Respir. Care 51, 29–35 (2006).

PubMed Google Scholar

Dawson, J. et al. Comparison of heart rate and oxygen saturation measurements from M asimo and N ellcor pulse oximeters in newly born term infants. Acta Paediatr. 102, 955–960 (2013).

Article CAS PubMed Google Scholar

Kim, E. H. et al. Accuracy of pulse oximeters at low oxygen saturations in children with congenital cyanotic heart disease: An observational study. Pediatr. Anesth. 29, 597–603 (2019).

Article Google Scholar

Nogueira, M. S. et al. Evaluation of wavelength ranges and tissue depth probed by diffuse reflectance spectroscopy for colorectal cancer detection. Sci. Rep. 11, 1–17 (2021).

Article CAS Google Scholar

Volynskaya, Z. I. et al. Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy. J. Biomed. Opt. 13, 024012 (2008).

Article ADS PubMed Google Scholar

Soto-Bernal, J. J. et al. Optical method to characterize and assess setting evolution of cement pastes. Appl. Opt. 59, D1–D5 (2020).

Article ADS CAS PubMed Google Scholar

Elliott, C., Vijayakumar, V., Zink, W. & Hansen, R. National instruments LabVIEW: A programming environment for laboratory automation and measurement. JALA 12, 17–24 (2007).

Google Scholar

Kirkman, I. & Buksh, P. Data acquisition and control using National Instruments’ “LabVIEW” software. Rev. Sci. Instrum. 63, 869–872 (1992).

Article ADS Google Scholar

Toh, T. S., Dondelinger, F. & Wang, D. Looking beyond the hype: Applied AI and machine learning in translational medicine. EBioMedicine 47, 607–615 (2019).

Article PubMed PubMed Central Google Scholar

Vanneschi, L. & Castelli, M. Multilayer Perceptrons (Elsevier, 2019).

Book Google Scholar

Park, Y., Cho, K. H., Park, J., Cha, S. M. & Kim, J. H. Development of early-warning protocol for predicting chlorophyll-a concentration using machine learning models in freshwater and estuarine reservoirs, Korea. Sci. Total Environ. 502, 31–41 (2015).

Article ADS CAS PubMed Google Scholar

Faber, D. J. et al. Oxygen saturation-dependent absorption and scattering of blood. Phys. Rev. Lett. 93, 028102 (2004).

Article ADS PubMed Google Scholar

Prahl, S. Optical Absorption of Hemoglobin. http://omlc.ogi.edu/spectra/hemoglobin (1999).

Lamola, A. A. & Russo, M. Fluorescence excitation spectrum of bilirubin in blood: A model for the action spectrum for phototherapy of neonatal jaundice. Photochem. Photobiol. 90, 294–296 (2014).

Article CAS PubMed Google Scholar

Park, C., Took, C. C. & Seong, J.-K. Machine learning in biomedical engineering. Biomed. Eng. Lett. 8, 1–3 (2018).

Article PubMed PubMed Central Google Scholar

Nasteski, V. An overview of the supervised machine learning methods. Horizons. B 4, 51–62 (2017).

Article Google Scholar

Jones, S., Carley, S. & Harrison, M. An introduction to power and sample size estimation. Emerg. Med. J. 20, 453 (2003).

Article CAS PubMed PubMed Central Google Scholar

Dutta, D. et al. Influence of admission weight on neonatal mortality amongst hospitalised neonates in Calcutta. J. Indian Med. Assoc. 90, 308–309 (1992).

CAS PubMed Google Scholar

Hammer, G. P., du Prel, J.-B. & Blettner, M. Avoiding bias in observational studies: Part 8 in a series of articles on evaluation of scientific publications. Dtsch. Arztebl. Int. 106, 664 (2009).

PubMed PubMed Central Google Scholar

Garber, C. C. Jendrassik–Grof analysis for total and direct bilirubin in serum with a centrifugal analyzer. Clin. Chem. 27, 1410–1416 (1981).

Article CAS PubMed Google Scholar

Fares, A. K. Performance evaluation of two haematology analysers: The Sysmex KX-21 and the Beckman Coulter AC. T diff. Sysmex J. Int. 11, 6 (2001).

Google Scholar

Bénéteau-Burnat, B., Bocque, M.-C., Lorin, A., Martin, C. & Vaubourdolle, M. Evaluation of the blood gas analyzer GEM (r) PREMIER (tm) 3000. Clin. Chem. Lab. Med. 42, 96–101 (2004).

Article PubMed Google Scholar

Kanagasabapathy, A. & Rao, P. Laboratory accreditation-procedural guidelines. Indian J. Clin. Biochem. 20, 186 (2005).

Article CAS PubMed PubMed Central Google Scholar

Polley, N. et al. Development and optimization of a noncontact optical device for online monitoring of jaundice in human subjects. J. Biomed. Opt. 20, 067001 (2015).

Article ADS PubMed Google Scholar

Bland, J. M. & Altman, D. Statistical methods for assessing agreement between two methods of clinical measurement. The Lancet 327, 307–310 (1986).

Article Google Scholar

Bland, J. M. & Altman, D. G. Correlation, regression, and repeated data. BMJ 308, 896 (1994).

Article CAS PubMed PubMed Central Google Scholar

Halder, A. et al. Large scale validation of a new non-invasive and non-contact bilirubinometer in neonates with risk factors. Sci. Rep. 10, 1–14 (2020).

Article Google Scholar

G. A. o. t. W. M. Association. World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects,". J. Am. Coll. Dentists 81, 14–18 (2014).

Google Scholar

Jung, Y. H. et al. The efficacy of noninvasive hemoglobin measurement by pulse CO-oximetry in neonates. Pediatr. Crit. Care Med. 14, 70–73 (2013).

Article PubMed Google Scholar

Shah, N., Ragaswamy, H. B., Govindugari, K. & Estanol, L. Performance of three new-generation pulse oximeters during motion and low perfusion in volunteers. J. Clin. Anesth. 24, 385–391 (2012).

Article PubMed Google Scholar

Nicholas, C. et al. Validation of noninvasive hemoglobin measurement by pulse co-oximeter in newborn infants. J. Perinatol. 35, 617–620 (2015).

Article CAS PubMed Google Scholar

Kazanasmaz, H. & Demir, M. The Comparison of hemoglobin values measured by blood and continuous non-invasive monitoring (SpHb) in newborn infants. J. Trop. Pediatr. 67, 050 (2021).

Article Google Scholar

Maisels, M. J. et al. Evaluation of a new transcutaneous bilirubinometer. Pediatrics 113, 1628–1635 (2004).

Article PubMed Google Scholar

Olusanya, B. O. et al. Management of late-preterm and term infants with hyperbilirubinaemia in resource-constrained settings. BMC Pediatr. 15, 1–12 (2015).

Article Google Scholar

Sedaghat-Yazdi, F., Torres, A. Jr., Fortuna, R. & Geiss, D. M. Pulse oximeter accuracy and precision affected by sensor location in cyanotic children. Pediatr. Crit. Care Med. 9, 393–397 (2008).

Article PubMed Google Scholar

Ross, P. A., Newth, C. J. & Khemani, R. G. Accuracy of pulse oximetry in children. Pediatrics 133, 22–29 (2014).

Article PubMed Google Scholar

Bhutani, V. K. et al. Noninvasive measurement of total serum bilirubin in a multiracial predischarge newborn population to assess the risk of severe hyperbilirubinemia. Pediatrics 106, e17–e17 (2000).

Article CAS PubMed Google Scholar

El-Beshbishi, S. N., Shattuck, K. E., Mohammad, A. A. & Petersen, J. R. Hyperbilirubinemia and transcutaneous bilirubinometry. Clin. Chem. 55, 1280–1287 (2009).

Article CAS PubMed Google Scholar

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SKP thanks the Indian National Academy of Engineering (INAE) for the Abdul Kalam Technology Innovation National Fellowship, INAE/121/AKF. The authors would like to acknowledge the Deanship of Scientific Research at Umm Al-Qura University, for supporting this work by Grant code: 22UQU4320545DSR31. Dr. Ziad Moussa is grateful to the United Arab Emirates University (UAEU) and to the Research Office for supporting the research developed in his laboratory and reported herein (SUREPLUS Grant code G00003918).

These authors contributed equally: Amrita Banerjee and Neha Bhattacharyya.

Department of Physics, Jadavpur University, 188, Raja S.C. Mallick Rd, Kolkata, 700032, India

Amrita Banerjee & Subhadipta Mukhopadhyay

Technical Research Centre, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata, West Bengal, 700106, India

Amrita Banerjee, Lopamudra Roy & Mahasweta Goswami

Department of Paediatric Medicine, Nil RatanSircar Medical College & Hospital, 138, AJC Bose Road, Sealdah, Raja Bazar, Kolkata, 700014, India

Amrita Banerjee, Annie Bajaj, Nilanjana Ghosh & Asim Kumar Mallick

Department of Radio Physics and Electronics, University of Calcutta, 92, Acharya Prafulla Chandra Rd, Machuabazar, Kolkata, 700009, India

Neha Bhattacharyya & Pulak Mondal

Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector 3, Salt Lake, Kolkata, 700106, India

Neha Bhattacharyya, Ria Ghosh, Soumendra Singh, Aniruddha Adhikari, Susmita Mondal & Samir Kumar Pal

Neo Care Inc, 27, Parker St, Dartmouth, NS, B2Y 2W1, Canada

Soumendra Singh

Electrical and Computer Engineering Department, Dalhousie University, 6299 South St, Halifax, NS B3H 4R2, Halifax, Canada

Soumendra Singh

Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA

Aniruddha Adhikari

Department of Applied Optics and Photonics, JD-2, Sector-III, Salt Lake, Kolkata, West Bengal, 700 106, India

Lopamudra Roy

Department of Biotechnology, Thapar Institute of Engineering and Technology, Bhadson Road, Patiala, Punjab, 147004, India

Aman Bhushan

Faculty of Medicine, Assiut University, Assiut, 71516, Egypt

Ahmed S. A. Ahmed

Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, P.O. Box 15551, Abu Dhabi, United Arab Emirates

Ziad Moussa

Department of Gynecology & Obstetrics, Nil Ratan Sircar Medical College & Hospital, 138, AJC Bose Road, Sealdah, Raja Bazar, Kolkata, 700014, India

Debasis Bhattacharyya

Department of Basic Science and Humanities, Techno International, Kolkata, 700156, India

Arpita Chattopadhyay

Department of Physics, Sister Nivedita University, Kolkata, India

Arpita Chattopadhyay

Department of Chemistry, Faculty of Applied Science, Umm Al-Qura University, Makkah, 21955, Saudi Arabia

Saleh A. Ahmed

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All authors wrote the main manuscript text, prepared figures and reviewed the manuscript.

Correspondence to Saleh A. Ahmed, Asim Kumar Mallick or Samir Kumar Pal.

The authors declare no conflict of interest. Soumendra Singh was a part of Technical Research Centre, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata, West Bengal 700106, India during the completion of the project. Currently he is associated with Neo Care Inc,27, Parker St, Dartmouth, NS B2Y2W1, Canada. In addition, the authors declare no competing interests.

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Banerjee, A., Bhattacharyya, N., Ghosh, R. et al. Non-invasive estimation of hemoglobin, bilirubin and oxygen saturation of neonates simultaneously using whole optical spectrum analysis at point of care. Sci Rep 13, 2370 (2023). https://doi.org/10.1038/s41598-023-29041-w

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Received: 29 September 2022

Accepted: 30 January 2023

Published: 09 February 2023

DOI: https://doi.org/10.1038/s41598-023-29041-w

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